Bond Elut C18 + EWP + OH
Bond Elut C18 SPE from Agilent Technologies
Bond Elut C18 is the most hydrophobic, bonded silica sorbent available in the Bond Elut range. It is the most popular SPE sorbent because of its extreme retentive nature for non-polar compounds. C18 is generally regarded as having the broadest spectrum of retention among bonded silica sorbents, since it retains most organic analytes from aqueous matrices. When analyzing small to intermediate molecules, Bond Elut C18 can be used for desalting aqueous matrices prior to ion exchange, as salts pass through the sorbent unretained.
Bond Elut EWP is based upon standard particles size silica but with 500 pores to allow more efficient extraction of large molecules (>15,000 MW), which are typically excluded from standard porosity silica phases.
Bond Elut C18 OH is a non-endcapped version of the octadecyl bonded phases that enables the silanols on the silica surface to be more active. This low-load C18 has well-controlled silanol activity that permits the fractionation of metabolites and enhances retention of basic compounds compared to an endcapped C18. The pore size makes it a good choice for peptide extraction.
All pictures shown are for illustration purpose only. Actual product may vary.
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- Features
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Bond Elut C18
- Extremely retentive for non-polar compounds
- Effective for desalting aqueous mixtures
- he most hydrophobic, bonded silica sorbent
Bond Elut EWP
- No exclusion of large molecules
- Good for desalting proteins
- Successful separation of proteins, peptides or nucleotides
Bond Elut OH
- Silanol activity permits metabolite fractionation
- Tight QC tolerances deliver batch-to-batch reproducibility
- 150 pore size expands retention window to higher molecular weight compounds
- Specifications
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Bond Elut C18 Bond Elut C18 EWP Bond Elut C18 OH Category Non-polar Bonded Functional Group Trifunctional octadecyl Trifunctional octadecyl Monofunctional octadecyl Base Material Silica based Endcapped Yes Yes No Format Packed bed Typical Carbon Loading 17.4% 6% 14.9% Surface Area 500 m2/g 80 m2/g 300 m2/g Particle Size 40 and 120 µm, irregular 40 µm, irregular 40 and 120 µm, irregular Mean Pore Size 60 Å 500 Å 150 Å - Literature
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Agilent Bond Elut Silica Brochure
All Silica based SPE phases in the Bond Elut Range from Agilent
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Bond Elut Family of SPE Products
Overview of Agilent's Bond Elut product range
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Agilent Sample Preparation Catalogue
Consumable items for sample preparation from Agilent
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